| 货号 | 12439S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:1200) |
| 供应商 | CST |
| 背景 | CD82 (KAI1) belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). CD82 does not have enzymatic activity and appears to function by regulating the trafficking of other proteins and organization of the cell membrane (1). CD82 was originally described as a costimulator for T cells that directly associates with CD4 and CD8, and was subsequently identified during a screen as a metastasis suppressor in prostate cancer (2,3). CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies (4-6). CD82 suppresses metastasis through multiple mechanisms including inhibition of cell motility and invasion by modulating c-Met and the urokinase plasminogen activator surface receptor (uPAR), as well as promotion of homotypic cell-cell adhesion by stabilizing interactions between E-cadherin and β-catenin (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 30-60 |
Western blot analysis of extracts from various cell lines using CD82 (D7G6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD82 (hCD82-Myc/DDK; +), using CD82 (D7G6H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using CD82 (D7G6H) | |
Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded breast adenocarcinoma using CD82 (D7G6H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using CD82 (D7G6H) Rabbit mAb. |
