| 货号 | 2895S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/ChIP |
| 目标/特异性 | CHOP (L63F7) Mouse mAb detects endogenous levels of total CHOP protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:3200) ChIP (1:200) |
| 供应商 | CST |
| 背景 | CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Confocal immunofluorescent analysis of A-204 cells, untreated (left) or tunicamycin-treated (right), using CHOP (L63F7) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 对A-204细胞，未处理（左）或tunicamycin处理（右）使用CHOP (L63F7) Mouse mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。 | |
Western blot analysis of extracts from C6 and A-204 cells, untreated or treated with thapsigargin (300 nM, 2 hours) or tunicamycin (24 μg/ml, 2 hours), using CHOP (L63F7) Mouse mAb. 未处理或者加入thapsigargin (300 nM，2小时)或tunicamycin (24 ug/ml，2小时)处理C6和A204细胞，使用CHOP (L63F7) Mouse mAb进行Western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and 1 µl of CHOP (L63F7) Mouse mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
