| 货号 | 9165S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) IF-IC (1:50) IHC-F (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 43, 48 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织，使用的抗体为c-Jun (60A8) Rabbit mAb。 | |
Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb.Western blot检测细胞提取物：未经处理和用紫外照射的NIH/3T3 和SK-N-MC 细胞，是用的抗体是c-Jun (60A8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人卵巢癌组织，使用的抗体为c-Jun (60A8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人星形细胞瘤 组织，使用的抗体为c-Jun (60A8) Rabbit mAb。 | |
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的冷冻H1650移植瘤组织，显示出细胞核的位置。使用的抗体为c-Jun (60A8) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, using c-Jun (60A8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，使用的抗体是c-Jun (60A8) Rabbit mAb (绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of c-Jun (60A8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
