| 货号 | 4573S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αPix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool2/αPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain (2). X-chromosomal genes mutated in nonspecific mental retardation (MRX) comprise a family of genes, including the gene encoding Cool2/αPix, thought to be involved in mental retardation (3,4). Cool2/αPix interacts with β-parvin/affixin, a protein involved in integrin signaling (5), and may act downstream of integrin-linked kinase (ILK) to regulate actin reorganization and cell spreading (6). When Cool2αPix exists as a dimer, it functions as a Rac-specific GEF, whereas the monomeric protein acts as a GEF for both Rac and Cdc42. Regulation of Cool2/αPix dimerization, and therefore its specificity, occurs at least in part through p21 activated kinase (PAK) in response to extracellular signaling (7). Further, binding of Cdc42 enhances the Rac GEF activity of the Cool2/αPix dimer. Activated Rac in turn inhibits Cool2/αPix Rac GEF activity (8).Cool/Pix蛋白是由定位于粘着斑的鸟嘌呤核苷交换因子（guanine nucleotide exchange factors，GEFs）家族组成。该家族包含有两个亚型：cool2/αpix and cool1/βPix，后者有两个剪切变异体，其区别是在它们的羧基终端(1)。像其它GEFs，Cool2/αPix蛋白有一个DH (Dbl homology)功能域，其能允许小GTPases的结合和GDP/GTP的交换，和一个PH (Pleckstrin homology) 功能域(2)。在非特异性精神发育迟滞（ nonspecific mental retardation，MRX）中X-chromosomal突变的基因包含一个基因家族，包括编码Cool2/αPix的基因，被认为涉及精神发育迟滞(3,4)。Cool2/αPix蛋白与β-parvin/affixin蛋白相互作用，而 β-parvin/affixin蛋白涉及整合蛋白信号(5)，并且Cool2/αPix蛋白可能作用integrin-linked kinase (ILK)的下游去调节actin 蛋白的重组和细胞铺展(6)。当Cool2αPix蛋白以一个二聚体存在，它的功能作为一个Rac-specific GEF，然而单体存在的蛋白对于Rac和Cdc42蛋白作为一个GEF。在细胞外信号反应下，通过p21 activated kinase (PAK)Cool2/αPix蛋白二聚化的调节和它的特异性在某种程度上发生(7)。进一步，Cdc42蛋白的结合提高Cool2/αPix二聚体的Rac GEF 活性。活化的Rac轮流地抑制Cool2/αPix Rac GEF活性(8)。 |
| 存放说明 | -20C |
| 计算分子量 | 87 |
Western blot analysis of extracts from various cell types using Cool2/αPix (C23D2) Rabbit mAb.使用Cool2/αPix (C23D2) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞系中Cool2/αPix (C23D2)蛋白水平。 | |
Confocal immunofluorescent analysis of RAW 264.7 (left) and SH-SY5Y cells (right) using Cool2/alphaPix (C23D2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用Cool2/alphaPix (C23D2) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析RAW 264.7 (左图)和SH-SY5Y细胞(右图)。DY-554 phalloidin标记微丝（红色）。蓝色= DRAQ5™（DNA荧光染料）。 |
