c-Rel (D5G1A) Rabbit mAb (ChIP Formulated)
货号：
12659S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12659S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP |
| 使用方法 | ChIP(1:50) |
性能| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| 存放说明 | -20C |
参考图片Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106Jurkat cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (50ng/ml) and Ionomycin, Calcium Salt #9995 (200ng/ml) and either 5 μl of c-Rel (D5G1A) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human BCL2L1 promoter primers, SimpleChIP® Human EP300 Promoter Primers #12281, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
