| 货号 | 4153S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/ChIP |
| 目标/特异性 | EGR1 (15F7) Rabbit mAb detects endogenous levels of total EGR1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:800) ChIP (1:50) |
| 供应商 | CST |
| 背景 | EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb. Western blot分析PC12细胞提取液，不处理或100 ng/ml NGF转染2小时，使用的抗体是EGR1 (15F7)Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). 共聚焦免疫荧光分析分离的PC12细胞，未经处理（左图）或经过NGF处理2小时（右图），使用的抗体是EGR1 (44D5) Rabbit mAb 兔单抗（绿色）和MEK1/2 (L38C12) Mouse mAb 鼠单抗#4694（红色）。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑，使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb. 免疫组化分析石蜡包埋的人神经母细胞瘤，使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗，显示出核定位。 | |
Immunohistochemical analysis of paraffin-embeded human Non-Hodgkins lymphoma, using EGR1 (15F7) Rabbit mAb. 免疫组化分析石蜡包埋的人非霍奇金淋巴瘤，使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗。 | |
