| 货号 | 2929S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | EpCAM (VU1D9) Mouse mAb detects endogenous levels of total EpCAM protein. |
| 使用方法 | WB(1:1000) IHC-P (1:500) F (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用EpCAM（VU1D9）小鼠单抗（绿色）对HT-29细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用Alexa Fluor® 555鬼笔环肽（红色）标记。蓝色假色=DRAQ5® #4084（DNA荧光染料）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using EpCAM (VU1D9) Mouse mAb.使用EpCAM（VU1D9）小鼠单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon using EpCAM (VU1D9) Mouse mAb.使用EpCAM（VU1D9）小鼠单抗对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).使用EpCAM（VU1D9）小鼠单抗（蓝色）对比非特异性对照抗体（红色）对非渗透性HT-29细胞进行流式细胞分析。 | |
Western blot analysis of extracts from HT-29 and HCT-15 cells using EpCAM (VU1D9) Mouse mAb.用EpCAM（VU1D9）小鼠单抗对HT-29和HCT-15细胞提取物进行western blot分析。 |
