| 货号 | 13826S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors include three protein receptors, ERRα/NR3B1, ERRβ/NR3B2, and ERRγ/NR3B3, that have yet to be associated with natural ligands. PGC-1 coactivators regulate ERR transcription activation ability and receptor-induced transcription of genes involved in lipid metabolism, glucose metabolism, and mitochondrial biogenesis (1). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hepa 1-6 cells and either 10 µl of ERRα (E1G1J) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse ESRRA promoter primers, mouse IDH3A intron 2 primers, SimpleChIP® Mouse OGDH Intron 1 Primers #13828, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using ERRα (E1G1J) Rabbit mAb. | |
Western blot analysis of extracts from BT-474 cells, untreated (-) or XCT790-treated (5 μM, 24 hr; +), using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). XCT790 is a selective inverse agonist of ERRα that induces its proteasomal degradation (10,11). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human ERRα protein (hERRα-Myc/DDK; +), full-length human ERRβ protein (hERRβ-Myc/DDK; +); full-length human ERRγ protein (hERRγ-Myc/DDK; +), full-length human ERα protein (ERα-Myc/DDK; +), or full-length human ERβ protein (hERβ-Myc/DDK; +), using ERRα (E1G1J) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). | |
Western blot analysis of heart tissue extracts from ERRα wildtype (+/+) and ERRα knockout (-/-) mice using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Heart tissue was kindly provided by Dr. Vincent Giguere of McGill University. |
