| 货号 | 2402S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | HSP27 (G31) Mouse mAb detects endogenous levels of total HSP27 protein. The antibody does not cross-react with other heat shock proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Western blot analysis of extracts from HeLa and COS-7 cells using HSP27 (G31) Mouse mAb. 对HeLa细胞和COS-7细胞使用HSP27 (G31) Mouse mAb进行Western blot。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left panels) or lambda phosphatase-treated (right panels), using Phospho-HSP27 (Ser82) Antibody #2401 (upper panels) or HSP27 (G31) Mouse mAb (lower panels). 对石蜡包埋的人乳腺癌，未处理（左）或用γ磷酸酶处理（右），使用Phospho-HSP27 (Ser82) Antibody #2401 (上) 或 HSP27 (G31) Mouse mAb(下)进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP27 (G31) Mouse mAb. 对石蜡包埋的人肺癌使用HSP27 (G31) Mouse mAb进行免疫组化分析。 | |
Confocal immunofluorescent analysis of A549 cells using HSP27 (G31) Mouse mAb (green). Red = Propidium iodide (fluorescent DNA dye). 对A549细胞使用HSP27 (G31) Mouse mAb（绿色）进行共聚焦免疫荧光分析。红色=Propiduim iodide (荧光DNA染料)。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® HSP27 siRNA I (+) using HSP27 (G31) Mouse mAb and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The HSP27 (G31) Mouse mAb confirms silencing of HSP27 expression, while the p44/42 MAPK (Erk1/2) (3A7) Mouse mAb is used to control for loading and specificity of HSP27 siRNA. 对转染100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或HSP27 siRNA I (+)的HeLa细胞使用HSP27 (G31) Mouse mAb和p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107进行Western blot分析。 HSP27 (G31) Mouse mAb证实HSP27的表达沉默，p44/42 MAPK (Erk1/2) (3A7) Mouse mAb用作显示HSP27 siRNA上样和特异性的对照。 |
