| 货号 | 5628S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | IRF-8 (D20D8) Rabbit mAb detect endogenous levels of total IRF-8 protein. An unknown background band is detected at 80 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:25) |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 and either 20 μl of IRF-8 (D20D8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human GPR18 promoter primers, human MS4A7 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。 THP-1细胞培养至4 x 106，然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,所用的抗体为20 μl IRF-8 (D20D8) Rabbit mAb或2 μl of Normal Rabbit IgG #2729，本实验中用 human GPR18 promoter primers, human MS4A7 promoter primers和 SimpleChIP® Human α Satellite Repeat Primers #4486引物对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 | |
Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml; overnight) using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究未经处理的和经hIFN-α1 #8927 (10 ng/ml)处理过夜的Raji 和 Jurkat细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1, RL7, and A20 cell lines using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究THP-1, RL7 和 A20细胞系的细胞提取液。 | |
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human and mouse IRF-8 constructs, using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究对照转染的和转染了人和小鼠IRF-8质粒的293T细胞的细胞提取液。 |
