| 货号 | 13594S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Western blot analysis of extracts from F9 cells and NTERA-2 cells, untreated (-) or treated with retinoic acid (1 μM, 5 d; +) to induce differentiation, using JARID2 (D6M9X) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, JARID2 protein levels decrease upon NTERA-2 cell differentiation. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 µl of JARID2 (D6M9X) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
