| 货号 | 3558S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | LDHA/LDHC (C28H7) Rabbit mAb detects endogenous levels of total LDHA and LDHC proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1-α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell types using LDHA/LDHC (C28H7) Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为LDHA/LDHC (C28H7) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LDHA/LDHC (C28H7) Rabbit mAb. 免疫组化检测人结肠癌组织石蜡切片，所用抗体为 LDHA/LDHC (C28H7) Rabbit mAb兔单抗. |
