| 货号 | 12159S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | LLGL1 (D2B5A) Rabbit mAb recognizes endogenous levels of total LLGL1 protein. This antibody does not cross-react with LLGL2. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | In Drosophila, lethal giant larvae (lgl), discs large (dlg), and scribble (scrib) act as tumor suppressor genes. Their loss of function in flies causes neoplastic overgrowth of larval brain tissue and imaginal epithelial cells hallmarked by disruption of the cytoskeletal network and cellular polarity (1,2). The human homolog of the Drosophila lgl protein, lethal giant larvae protein homolog 1 (LLGL1), is a cytoskeletal protein implicated in regulating cellular organization, migration, and cell polarity (3). As in Drosophila, decreased expression of LLGL1 correlates with an increased incidence of cellular overgrowth and malignant transformation (4-6). In mammalian epithelial cells, LLGL1 redistributes from the cytoplasm to regions of cell-cell contact, allowing the establishment and maintainence of a polarized morphology (7). LLGL1 also plays a role in the formation of epithelial junctions via its direct interactions with PAR6 and aPKC, the latter of which has been shown to phosphorylate LLGL1 at Ser663, thus restricting its localization to the basolateral region of the cell (8). LLGL1 may also play an additional, unrealized role in cellular development and differentiation as indicated by the fact that Drosophila lgl has been implicated in controlling self-renewal and differentiation of progenitor cells (9). Recent studies in mice have suggested that a mammalian LLGL1 homolog that does not have tumor suppressor-like acitvity, LLGL2, is required for proper polarized invasion of trophoblasts and efficient branching morphogenesis during placental development (10). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of OVCAR3 (positive; left) and Hep3B (weak; right) cells using LLGL1 (D2B5A) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用LLGL1 (D2B5A) Rabbit mAb（绿色）对OVCAR3 (阳性；左) 和 Hep3B (弱阳性；右)细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using LLGL1 (D2B5A) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).使用LLGL1 (D2B5A) Rabbit mAb（上）或GAPDH (D16H11) XP® Rabbit mAb #5174（下）对多种细胞提取物进行western blot分析。 |
