| 货号 | 12455S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | MacroH2A1.1 (D5F6N) Rabbit mAb recognizes endogenous levels of total MacroH2A1.1 protein. This antibody does not cross-react with other MacroH2A proteins, including MacroH2A1.2 and MacroH2A2. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal “macro” domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to locally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Confocal immunofluorescent analysis of HeLa cells using MacroH2A1.1 (D5F6N) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 采用共聚焦免疫荧光术检测Hela细胞（左）和NIH/3T3细胞（右），使用的抗体为MacroH2A1.1 (D5F6N) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝使用DY-554 鬼笔环肽进行标记（红色）。 | |
Western blot analysis of extracts from various tissues using MacroH2A1.1 (D5F6N) Rabbit mAb. Western blot方法检测不同细胞系的提取物，所用抗体为MacroH2A1.1 (D5F6N) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MacroH2A1.1 (hMacroH2A1.1-DDK; +) or DDK-tagged full-length human MacroH2A1.2 (hMacroH2A1.2-DDK; +), using MacroH2A1.1 (D5F6N) Rabbit mAb (upper) and DYKDDDDK Tag (9A3) Mouse mAb #8146 (lower). Western blot方法检测293细胞提取物，分别用模拟物（-）或构建的带有Myc/DDK标签的全长人MacroH2A1.1 (hMacroH2A1.1-Myc/DDK; +)、或带 DDK标签的全长人MacroH2A1.2 (hMacroH2A1.2-DDK; +)，使用的抗体为 MacroH2A1.1 (D5F6N) Rabbit mAb 兔单抗(上图) 以及 DYKDDDDK Tag (9A3) Mouse mAb #8146 鼠单抗(下图)。 |
