| 货号 | 12166S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | MAML1 (D3K7B) Rabbit mAb recognizes endogenous levels of total MAML1 protein. This antibody does not detect MAML2 or MAML3. |
| 使用方法 | WB(1:1000) IP (1:200) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthins tumors (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from various cell lines using MAML1 (D3K7B) Rabbit mAb. 使用MAML1 (D3K7B)Rabbit mAb对多种细胞系提取物进行western blot分析。 | |
Immunoprecipitation of MAML1 protein from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MAML1 (D3K7B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MAML1 Antibody #4608. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或MAML1 (D3K7B)Rabbit mAb兔单抗（lane3）对293T细胞中的MAML1蛋白进行免疫沉淀实验。Lane1是10%上样量。使用MAML1抗体#4608进行western blot分析。 | |
CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM) for 3 days and then washed and cultured in fresh media for 3 h. Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of MAML1 (D3K7B) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human DTX1 intron 3 primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUTLL1细胞在含有γ-分泌酶抑制剂（1 μM）的培养基中培养三天，清洗后在正常培养基中培养3h。使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，结合5 µl of MAML1 (D3K7B) Rabbit mAb兔单抗或2 µl Normal Rabbit IgG #2729对已交联的4 x 106细胞的染色质样品进行染色质免疫沉淀实验。 使用human DTX1 intron 3 引物 ，SimpleChIP® Human HES4 Promoter 引物#7273, 和SimpleChIP® Human α Satellite Repeat 引物 #4486对富集的DNA样品进行real-time PCR的定量分析。每个样品的免疫沉淀的DNA量是相对于Input染色质的相对定量，Input染色质量设定值为1。 |
