| 货号 | 13475S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Melanoma cell adhesion molecule (MCAM, MUC18, CD146) is an immunoglobulin superfamily member originally described as a cell surface adhesion protein and marker of the progression and metastasis of melanoma (1,2). Expression of MCAM protein is seen in vascular endothelial cells, activated T lymphocytes, smooth muscle, and bone marrow stromal cells. Research studies demonstrate increased MCAM expression in endothelial cells from angiogenesis-related disorders, including inflammatory bowel disease, Crohn’s disease, rheumatoid arthritis, tumors, and chronic renal failure (3). MCAM-expressing human mesenchymal stromal cells (hMSC) in the hematopoietic microenvironment are responsible for maintaining the self-renewal of hematopoietic stem and progenitor cells (HSPC) through direct contact between hMSC and those cells (2). Related studies suggest that activation of the Notch signaling pathway may also, in part, play a role in HSPC maintenance (4). Additional research indicates that MCAM may play a role in multiple sclerosis, an autoimmune inflammatory disease that affects central nervous system neurons. Endothelial MCAM within the blood-brain barrier act as adhesion receptors that permit lymphocytes to transmigrate across the barrier and produce the inflammatory lesions that characterize the disorder (5). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Confocal immunofluorescent analysis of SK-MEL-28 (positive, left) and COLO 205 (negative, right) using MCAM (P1H12) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from SK-MEL-28, HUVEC, and COLO 205 cells using MCAM (P1H12) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, COLO 205 cells are negative for MCAM protein. | |
Immunoprecipitation of MCAM from HeLa cells using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or MCAM (P1H12) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MCAM (P1H12) Mouse mAb. |
