| 货号 | 13342S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | MDR1/ABCB1 (E1Y7B) Rabbit mAb recognizes endogenous levels of total MDR1 (ABCB1) protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | MDR1/ABCB1 belongs to the Mdr/Tap subfamily of the ATP-binding cassette transporter superfamily (1). Multidrug resistance 1 (MDR1) serves as an efflux pump for xenobiotic compounds with broad substrate specificity. MDR1 substrates include therapeutic agents such as actinomycin D, etoposide, imatinib, and doxorubicin, as well as endogenous molecules including β-amyloids, steroid hormones, lipids, phospholipids, cholesterol, and cytokines (2). Research studies have shown that MDR1 reduces drug accumulation in cancer cells, allowing the development of drug resistance (3-5). On the other hand, MDR1 expressed in the plasma membrane of cells in the blood-brain, blood-cerebral spinal fluid, or blood-placenta barriers restricts the permeability of drugs into these organs from the apical or serosal side (6,7). MDR1 is also expressed in normal tissues with excretory function such as small intestine, liver, and kidney (7). Intracellular MDR1 has been detected in the ER, vesicles, and nuclear envelope, and has been associated with cell trafficking machinery (8). Other reported functions of MDR1 include viral resistance, cytokine trafficking (9,10), and lipid homeostasis in the peripheral and central nervous system (11-13). |
| 存放说明 | -20C |
| 计算分子量 | 130-180 |
Confocal immunofluorescent analysis of HCT-15 (left) and MCF7 (right) cells using MDR1/ABCB1 (E1Y7B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用MDR1/ABCB1 (E1Y7B) Rabbit mAb兔单抗 (绿色)对HCT-15 (左)和MCF7 (右)进行激光共聚焦免疫荧光分析。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Immunoprecipitation of MDR1/ABCB1 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MDR1/ABCB1 (E1Y7B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MDR1/ABCB1 (E1Y7B) Rabbit mAb兔单抗. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 MDR1/ABCB1 (E1Y7B) Rabbit mAb (lane 3)对Hep G2细胞提取物中的MDR1/ABCB1进行免疫沉淀。Lane1是10%上样量。Western blot实验使用MDR1/ABCB1 (E1Y7B) Rabbit mAb兔单抗进行。 | |
Western blot analysis of extracts from various cell lines using MDR1/ABCB1 (E1Y7B) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. 使用MDR1/ABCB1 (E1Y7B) Rabbit mAb兔单抗 and β-Actin (13E5) Rabbit mAb 兔单抗#4970对多种细胞提取物进行western blot分析。 |
