| 货号 | 12590S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 目标/特异性 | MITF (D5G7V) Rabbit mAb recognizes endogenous levels of total MITF protein. The antibody is predicted to recognize all known isoforms. |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression of cell and tissue types (6). |
| 存放说明 | -20C |
| 计算分子量 | 50-75 |
Western blot analysis of extracts from various cell lines using MITF (D5G7V) Rabbit mAb. 使用MITF (D5G7V) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 SK-MEL-28 cells and either 10 μl of MITF (D5G7V) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Tyrosinase Promoter Primers #12970, human VMD2 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 SK-MEL-28细胞与10μl的MITF (D5G7V) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human Tyrosinase Promoter 引物 #12970，human VMD2 intron 1 引物,和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相比input染色质总量进行相对定量，input染色质量设定值为1。 |
