| 货号 | 11925S |
| 反应种属 | Human/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Mitofusin-2 (D1E9) Rabbit mAb recognizes endogenous levels of total mitofusin-2 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Mitofusins are mitochondrial transmembrane GTPases that function to regulate mitochondrial fusion, a process that occurs in concert with mitochondrial division and is necessary for the maintenance of structural and genetic mitochondrial integrity (1,2). Two mitofusins have been described in mammals, mitofusin-1 and -2, which share 60% amino acid identity and appear to function coordinately to regulate mitochondrial fusion (3). Mitochondrial fusion is widely recognized as important for normal cell growth and development (4), and may have evolved as a mechanism to offset the deleterious effects of mtDNA mutations (3). Null mutations in either mitofusin are embryonic lethal in mice, whereas conditional knockout studies have shown that combined deletion of mitofusin-1 and mitofusin-2 in skeletal muscle results in severe mitochondrial dysfunction (3). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Confocal immunofluorescent analysis of HeLa cells using Mitofusin-2 (D1E9) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，使用抗体为Mitofusin-2 (D1E9) Rabbit mAb（绿色）。DY-554 phalloidin（红色）标记肌动蛋白微丝。蓝伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using Mitofusin-2 (D1E9) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为Mitofusin-2 (D1E9) Rabbit mAb。 | |
Immunoprecipitation of Mitofusin-2 from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Mitofusin-2 (D1E9) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Mitofusin-2 (D2D10) Rabbit mAb #9482. 免疫沉淀方法检测PANC-1细胞提取物线粒体融合蛋白2，使用抗体为Normal Rabbit IgG #2729 (泳道2)或Mitofusin-2 (D1E9) Rabbit mAb (泳道3)。泳道1为10%对照。使用Mitofusin-2 (D2D10) Rabbit mAb #9482进行Western blot分析。 |
