| 货号 | 12857S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | ABCC4 (D1Z3W) Rabbit mAb recognizes endogenous levels of total ABCC4 protein. This antibody cross-reacts with a nonspecific band at 47 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1200) |
| 供应商 | CST |
| 背景 | ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostaglandins (7). Research studies have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8,9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki disease, a childhood genetic disorder characterized by vasculitis (11). |
| 存放说明 | -20C |
| 计算分子量 | 140-200 |
Immunohistochemical analysis of paraffin-embedded cell pellets, HT-29 (left) and U-2 OS (right), using ABCC4 (D1Z3W) Rabbit mAb. 使用ABCC4 (D1Z3W) Rabbit mAb兔单抗对石蜡包埋的细胞进行免疫组化分析，HT-29 (左) 和 U-2 OS (右)。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ABCC4 (D1Z3W) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 在对照肽(左)或抗原特异性肽段(右)存在的条件下，使用ABCC4 (D1Z3W) Rabbit mAb兔单抗对石蜡包埋的人前列腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using ABCC4 (D1Z3W) Rabbit mAb. 使用ABCC4 (D1Z3W) Rabbit mAb兔单抗对石蜡包埋的人非小细胞肺癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from membrane fractions of SW620, LNCaP, and HT-29 cells using ABCC4 (D1Z3W) Rabbit mAb. 使用ABCC4 (D1Z3W) Rabbit mAb兔单抗对SW620, LNCaP, 和HT-29细胞膜提取物进行western blot分析。 |
