| 货号 | 2983S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | mTOR (7C10) Rabbit mAb detects endogenous levels of total mTOR protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:800) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 计算分子量 | 289 |
Flow cytometric analysis of 293 cells using mTOR (7C10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞技术分析293细胞；使用的抗体是 mTOR (7C10) Rabbit mAb兔单抗（蓝色），非特异性的阴性对照抗体（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using mTOR (7C10) Rabbit mAb. 免疫组织化学方法检测石蜡包埋的人乳腺癌组织，展示胞质定位；使用的抗体是mTOR (7C10) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using mTOR (7C10) Rabbit mAb in the presence of control peptide (left) or mTOR Blocking Peptide #1072 (right). 免疫组织化学方法检测石蜡包埋的人肺癌组织，左图是对照肽段处理的，右图是mTOR Blocking Peptide #1072处理的；使用的抗体是mTOR (7C10) Rabbit mAb兔单抗 。 | |
Western blot analysis of extracts from 293, A431, COS, C6, and C2C12 cells, using mTOR (7C10) Rabbit mAb. Western blot检测分析293, A431, COS, C6 和C2C12 细胞提取物。使用抗体是mTOR (7C10) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of mTOR siRNA. Western blot检测Hela细胞提取物，用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)和 SignalSilence® mTOR siRNA II (+)转染；使用的抗体是mTOR (7C10) Rabbit mAb 兔单抗#2983 和 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。mTOR (7C10) Rabbit mAb兔单抗 确定mTOR 表达的沉默效率, α-Tubulin (11H10) Rabbit mAb 兔单抗用于上样量和 mTOR siRNA特异性的对照。 | |
Confocal immunofluorescent analysis of mouse embryonic fibroblast (MEF) cells using mTOR (7C10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测鼠胚胎成纤维细胞（MEF），使用的抗体是mTOR (7C10) Rabbit mAb （绿色）。微丝用DY-554 phalloidin标记（红色）。蓝色伪彩是 DRAQ5® #4084 (fluorescent DNA dye)。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using mTOR (7C10) Rabbit mAb. |
