| 货号 | 8574S |
| 反应种属 | Human/Monkey/Dog |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | MYPT1 (D6C1) Rabbit mAb recognizes endogenous levels of total MYPT1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4). Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Confocal immunofluorescent analysis of Hep G2 cells using MYPT1 (D6C1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用MYPT1 (D6C1)Rabbit mAb（绿色）对HepG2细胞进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using MYPT1 (D6C1) Rabbit mAb.使用MYPT1 (D6C1)Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of MYPT1 from HeLa cell extracts using MYPT1 (D6C1) Rabbit mAb (lane 2). Western blot was performed using the same antibody. Lane 1 is 10% input.使用MYPT1 (D6C1)Rabbit mAb（Lane2）对HeLa细胞提取物中的MYPT1进行免疫沉淀分析。同样的抗体也被用于western blot分析。Lane是10%上样量。 |
