| 货号 | 4760S |
| 反应种属 | Rat |
| 来源宿主 | Mouse |
| 应用 | IHC-P/IF-F |
| 目标/特异性 | Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein. |
| 使用方法 | IHC-P(1:400) IF-F (1:300) |
| 供应商 | CST |
| 背景 | Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells (3). Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous. |
| 存放说明 | -20C |
| 计算分子量 | 207 |
Confocal immunofluorescent analysis of P1 rat brain (left) and adult rat brain (right) using Nestin (Rat-401) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析P1大鼠大脑（左）和成年大鼠大脑（右），使用的抗体是Nestin (Rat-401) Mouse mAb 鼠单抗（绿色）和Neurofilament-L (C28E10) Rabbit mAb 兔单抗#2837（红色）。蓝色伪彩=DRAQ5 ®#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded rat brain, P1 (left) or P14 (right), using Nestin (Rat-401) Mouse mAb (top) or Neurofilament-L (C28E10) Rabbit mAb #2837 (lower). Note decreased Nestin staining as NFL staining increases in the developing rat brain. 免疫组化分析石蜡包埋的大鼠大脑，P1（左）或P14（右），使用的抗体是Nestin (Rat-401) Mouse mAb 鼠单抗（上图）或Neurofilament-L (C28E10) Rabbit mAb 兔单抗#2837（下图）。注意：发育大鼠的脑中Nestin染色下降，NFL染色增加。 |
