| 货号 | 2836S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-F |
| 目标/特异性 | Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988). |
| 使用方法 | WB(1:1000) IP () IHC-P (1:200) IF-F (1:400) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).Studies of NFH (-/-) mice suggest that NFH modulates ion channel functions in large myelinated fibers (5). |
| 存放说明 | -20C |
| 计算分子量 | 180-220 |
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-H (RMdO 20) Mouse mAb. 免疫组化分析石蜡包埋的人脑，使用的抗体是Neurofilament-H (RMdO 20) Mouse mAb 鼠单抗。 | |
Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb. Western blot分析小鼠、大鼠或CAD细胞提取物，使用的抗体是Neurofilament-H (RMdO 20) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-H (RMdO 20) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠小脑，使用Neurofilament-H (RMdO 20) Mouse mAb 鼠单抗（绿）和Calbindin Antibody 兔多抗#2136（红）进行标记。蓝色伪彩=DRAQ5™(荧光DNA染料)。 |
