NF-κB1 p105/p50 (D7H5M) Rabbit mAb【科瑞奥博】

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NF-κB1 p105/p50 (D7H5M) Rabbit mAb

货号： 12540S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12540S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP/ChIP
目标/特异性	NF-κB1 p105/p50 (D7H5M) Rabbit mAb recognizes endogenous levels of total NF-κB1 p105/p50 protein.
使用方法	WB(1:1000) IP (1:100) ChIP (1:50)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
存放说明	-20C
计算分子量	50 Active form. 120 Precursor

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (30 ng/ml) for 1 hour and either 10 μl of NF-κB1 p105/p50 (D7H5M) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IAP2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HeLa细胞中的交联染色质，细胞通过Human Tumor Necrosis Factor-α (hTNF-α) #8902 (30 ng/ml) 处理1小时或者使用 10 μl of NF-κB1 p105/p50 (D7H5M) Rabbit mAb 或 2 μl of Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用SimpleChIP® Human IκBα Promoter Primers #5552, human IAP2 promoter primers, 和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀的DNA的数量形成与总数量的相对值。

Western blot analysis of extracts from various cell lines using NF-κB1 p105/p50 (D7H5M) Rabbit mAb.Western blot分析多种细胞系的细胞提取物，使用抗体是NF-κB1 p105/p50 (D7H5M) Rabbit mAb。

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml) for the indicated times, using NF-κB1 p105/p50 (D7H5M) Rabbit mAb.Western blot分析HeLa细胞的细胞提取物，未处理(-)或使用Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml)按指示时间处理，使用抗体是NF-κB1 p105/p50 (D7H5M) Rabbit mAb。