| 货号 | 75463S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Oct-4 (D7O5Z) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). As expected, HeLa cells are negative for Oct-4 expression. | |
Western blot analysis of extracts from NTERA-2 and HeLa cells using Oct-4 (D7O5Z) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells are negative for Oct-4 expression. | |
Flow cytometric analysis of HeLa cells (blue) and NTERA cells (green) using Oct-4 (D7O5Z) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 fragment (Alexa Fluor 488 conjugate) #4408 was used as a secondary Ab. |
