| 货号 | 5677S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP |
| 目标/特异性 | Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) detects endogenous levels of total Oct-4A protein. |
| 使用方法 | ChIP(1:50) |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | -20C |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 NCCIT细胞和10μl Oct-4A (C30A3C1)Rabbit mAb兔单抗（ChIP级别）或2μl Normal Rabbit IgG#2779进行染色质免疫共沉淀。富集到的DNA随后使用SimpleChIP® Human Oct-4启动子引物#4641，SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量显示比对input染色质总量进行相对定量，Input染色质量设定值为1。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 小鼠胚胎干细胞和10μl Oct-4A (C30A3C1)Rabbit mAb兔单抗（ChIP级别）或2μl Normal Rabbit IgG#2779进行染色质免疫共沉淀。富集到的DNA随后使用SimpleChIP®小鼠 Oct-4启动子引物#4653，SimpleChIP® Mouse XIST内含子1引物#4659和SimpleChIP®鼠RPL30内含子2引物#7015进行荧光实时PCR定量。各样品沉淀得到的DNA量显示比对input染色质总量进行相对定量，Input染色质量设定值为1。 |
