| 货号 | 2890S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | Oct-4A (C52G3) Rabbit mAb detects endogenous levels of total Oct-4A protein. |
| 使用方法 | WB(1:1000) IHC-P (1:1200) F (1:400) IF-IC (1:1600) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Confocal immunofluorescent analysis of NTERA2 cells using Oct-4A (C52G3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Oct-4A (C52G3) Rabbit mAb 兔单抗（绿色）对NTERA2细胞进行激光共聚焦荧光分析。使用DY-554 phalloidin（红色）标记肌动蛋白丝。 | |
Western blot analysis of extracts from NCCIT and NTERA2 cells using Oct-4A (C52G3) Rabbit mAb. 使用Oct-4A (C52G3) Rabbit mAb兔单抗对NCCIT和NTERA2细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human seminoma, showing nuclear localization, using Oct-4A (C52G3) Rabbit mAb. 使用Oct-4A (C52G3) Rabbit mAb兔单抗对石蜡包埋的人精原细胞瘤进行免疫组化分析，结果显示Oct-4A定位于细胞核。 | |
Immunohistochemical analysis of paraffin-embedded NTERA2 (positive, left) and HeLa (negative, right) cell pellets using Oct-4A (C52G3) Rabbit mAb. 使用Oct-4A (C52G3) Rabbit mAb兔单抗对石蜡包埋的NTERA2（阳性，左）和HeLa（阴性，右）细胞进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C52G3) Rabbit mAb. 使用Oct-4A (C52G3) 对HeLa细胞（蓝色）和NCCIT细胞（绿色）进行流式细胞分析。 | |
