| 货号 | 2407S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6).人类p14 ARF (小鼠中为p19 ARF)是一种促凋亡的细胞周期调节子，在人类肿瘤中经常性失活(1)。在大多数细胞中p14 ARF的基础表达水平是比较低的，但是当癌基因表达时会发生该蛋白的累积(2,3)。表达增加的p14 ARF促进MDM2的降解，导致p53蛋白水平的升高和随后的细胞周期捕获和(或)细胞凋亡(4)。尽管大多数p14 ARF信号传统意义上被认为是p53依赖的，但新近的报道表明p53非依赖的p14 ARF信号也引起细胞周期捕获和细胞凋亡(5,6)。 |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using p14 ARF (4C6/4) Mouse mAb.免疫组织化学方法检测石蜡包埋的人类前列腺癌组织，使用的抗体为p14 ARF（4C6/4）Mouse mAb. | |
Western blot analysis of HeLa cell lysate using p14 ARF (4C6/4) Mouse mAb.Western blot 方法检测Hela细胞裂解液，使用的抗体为 p14 ARF (4C6/4) Mouse mAb。 |
