| 货号 | 2586S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:2000) IP (1:100) IHC-P (1:4000) F (1:800) IF-IC (1:2400) |
| 供应商 | CST |
| 背景 | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation, and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.增殖细胞核抗原(PCNA)是DNA滑动压板蛋白家族的一员，该蛋白家族能够协助DNA复制(1)。晶体结构数据表明一个PCNA同源二聚体环能够沿着DNA双螺旋包绕和滑动(2)。多种蛋白参与了DNA复制和DNA修复，细胞周期控制结合PCNA而非直接联合DNA，从而促进了DNA的快速处理(3中已论述)。PCNA蛋白表达是一个公认的细胞增殖标志，而且PCNA (PC10)小鼠单抗已被用来评估众多研究中PCNA的水平。 |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类结肠癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类乳腺癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.免疫组织化学方法检测石蜡包埋人类非霍奇金淋巴瘤癌组织，显示核定位，使用的抗体为PCNA (PC10) Mouse mAb。 | |
Western blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967(lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2).Western blot 方法检测Hela细胞提取物（泳道1）和用PCNA (PC10) Mouse mAb 从同一提取物中免疫沉淀的PCNA（泳道2），使用的抗体为PCNA (PC10) Mouse mAb (上图) 和 Beta-Actin Antibody #4967(下图)。 | |
Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.Western blot 方法检测人类Hela细胞，鼠SV-T2细胞，牛BAEC细胞及猪PAE细胞提取物，使用的抗体为PCNA (PC10) Mouse mAb。 | |
Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (green), Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate) (red), and p21 Waf1/Cip1 (12D1) Rabbit mAb (blue).激光共聚焦免疫荧光方法检测NCI-H460细胞，使用的抗体为PCNA (PC10) Mouse mAb ，呈绿色，Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate)，呈红色，及 p21 Waf1/Cip1 (12D1) Rabbit mAb ，呈蓝色。 | |
Flow cytometric analysis of Jurkat cells using PCNA (PC10) Mouse mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. |
