| 货号 | 15616S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP ChIP |
| 目标/特异性 | SPT6 (D6J9H) Rabbit mAb recognizes endogenous levels of total SPT6 protein. |
| 使用方法 | WB(1:1000) IP (1:200) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SPT6 or SUPT6H is a histone H3 chaperone protein involved in transcriptional elongation and chromatin structure (1). The SPT6 protein contains a highly acidic N-terminus with leucine zipper and SH2 domains, which can interact with phospho-Rpb1 CTD (Ser2) to recruit SPN1 and other mRNA processing and export factors (2). SPT6 can enhance the elongation rate of RNA polymerase II, and can also maintain the modification state of histone H3 tails. (3-4). Loss of SPT6 causes improper initiation of transcription within coding regions (5). |
| 存放说明 | -20C |
| 计算分子量 | 210 |
| 参考文献 | 1 . Bortvin, A. and Winston, F. (1996) Science 272, 1473-6. 2 . Yoh, S.M. et al. (2007) Genes Dev 21, 160-74. 3 . Ardehali, M.B. et al. (2009) EMBO J 28, 1067-77. 4 . Kato, H. et al. (2013) Sci Rep 3, 2186. 5 . Kaplan, C.D. et al. (2003) Science 301, 1096-9. |
Western blot analysis of extracts from various cell lines using SPT6 (D6J9H) Rabbit mAb. | |
Immunoprecipitation of SPT6 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SPT6 (D6J9H) Rabbit mAb. Western blot analysis was performed using SPT6 (D6J9H) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of SPT6 (D6J9H) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Myc Intron Primers #14905, SimpleChIP® Human GAPDH Exon 1 Primers #5516, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
