| 货号 | 5524S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | PITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the CDK11 gene (1). CDK11A and CDK11B encode nearly identical serine/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34CDC2 family of protein kinases (2). Full-length PITSLRE/CDK11 (commonly referred to as CDK11p110) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11p58), the result of internal ribosomal entry, is expressed only during the G2/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE/CDK11 (CDK11p110) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11p110 interacts with numerous splicing factors including RNPS1, 9G8/SRSF7 and cyclin L and that CDK11p110 can phosphorylate and inhibit the splicing activity of 9G8/SRSF7 (9-11).PITSLRE，也称为细胞分裂激酶11（CDK11），是CDK11基因的复制产物(1)。CDK11A 和CDK11B能够分别编码几乎完全相同的蛋白激酶PITSLREB和PITSLREA，都属于p34CDC2蛋白激酶家族(2)。全长的PITSLRE/CDK11(通常称作CDK11p110)在整个细胞周期广泛表达，而只有在G2/M转换期表达较少且选择性转录(CDK11p58)-内部核糖体进入的结果，该转换期它能够促进中心体的成熟和纺锤体的形成(3-5)。在Fas或TNF诱导的细胞凋亡或者脱落凋亡中，PITSLRE/CDK11被凋亡蛋白酶剪切产生p110C-约46 kDa的蛋白，包含PITSLRE/CDK11的催化激活激酶结构域，该结构域能够与p21激活激酶(PAK1)相互作用并抑制其活性(6-8)。全长PITSLRE/CDK11 (CDK11p110)似乎参与了前体mRNA的剪接事件。同过观察CDK11p110与多种剪接因子包括RNPS1、9G8/SRSF7 和cyclin L的相互作用可以表明这一点，而且CDK11p110能够磷酸化和抑制9G8/SRSF7的剪接活性(9-11)。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Confocal immunofluorescent analysis of A-431 cells (left) and UACC62 cells (right) using PITSLRE/CDK11 (D88B3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测A-431细胞（左图）和UACC62细胞 （右图），使用的抗体为PITSLRE/CDK11 (D88B3) Rabbit mAb (绿色)。肌动蛋白纤维由DY-554 鬼笔环肽标记，呈红色。 | |
Western blot analysis of extracts from various cell lines using PITSLRE/CDK11 (D88B3) Rabbit mAb.Western blot 方法检测不同细胞提取物，使用的抗体为PITSLRE/CDK11 (D88B3) Rabbit mAb。 |
