PKCθ (E1I7Y) Rabbit mAb【科瑞奥博】

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PKCθ (E1I7Y) Rabbit mAb

货号： 13643S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	13643S
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F
使用方法	WB(1:1000) IP (1:100) IHC-P (1:50) F (1:400) IF-IC (1:400)

性能
供应商	CST
背景	PKCθ is a novel protein kinase C that is predominantly expressed in T cells (8). Recruitment of PKCθ to the immunological synapse following T cell receptor stimulation plays an important role in the activation and proliferation of conventional T cells (9). Conversely, PKCθ negatively regulates the suppressive function of regulatory T cells and is excluded from regulatory T cell immunological synapses (10).
存放说明	-20C
计算分子量	78

参考图片

Flow cytometric analysis of mouse splenocytes using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (left) and PKCθ (E1I7Y) Rabbit mAb (right). Splenocytes were co-stained with anti-CD3 APC and the Anti-rabbit IgG (H+L), F(ab)₂ Fragment (PE Conjugate) #8885 was used as a secondary antibody.

Confocal immunofluorescent analysis of Jurkat (positive, left) and Raji (negative, right) cells using PKCθ (E1I7Y) Rabbit mAb (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded Jurkat (positive, left) or RL7 (negative, right) cell pellets using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymph node using PKCθ (E1I7Y) Rabbit mAb.

Western blot analysis of extracts from various cell lines using PKCθ (E1I7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of PKCθ from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or PKCθ (E1I7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PKCθ (E1I7Y) Rabbit mAb.

Western blot analysis of extracts from wild-type and PKCθ (-/-) mouse splenocytes using PKCθ (E1I7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Extracts from wild-type and PKCθ (-/-) mouse splenocytes were kindly provided by Dr. Morgan Huse (Memorial Sloan-Kettering Cancer Center).