| 货号 | 13552S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Prolactin receptor (PRLR) is a single-pass transmembrane receptor that mediates the actions of prolactin, a peptide hormone secreted by the anterior pituitary. PRLR is a type 1 cytokine receptor that is best known for promoting lactation in mammals, but which is also implicated in osmoregulation, metabolism, and immune system function (1). Research studies suggest that PRLR activation may promote tumor growth (2). Prolactin signaling via PRLR can activate multiple signal transduction pathways in breast cancer cells, including the Jak/Stat, PI3K/Akt, and MAPK pathways, leading to both pro-proliferative and anti-apoptotic downstream effects (3,4). Nine isoforms of PRLR have been identified, with the canonical (long) isoform primarily responsible for the pro-oncogenic effects of PRLR in some cancer cell lines (3). Much less is known about the functions of the other prolactin receptor isoforms. Defining the precise role of PRLR in promoting growth of breast cancer and other tumor types remains an area of active investigation (2). |
| 存放说明 | -20C |
| 计算分子量 | 95, 65 |
Western blot analysis of extracts from MCF7 cells, transfected with SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence®Prolactin Receptor siRNA I #13822 (+) or SignalSilence® Prolactin Receptor siRNA II #13836 (+), using Prolactin Receptor (D4A9) Rabbit mAb (upper), or β-actin (D8A6) Rabbit mAb #8457 (lower). The Prolactin Receptor (D4A9) Rabbit mAb confirms silencing of Prolactin Receptor expression, while the β-actin (D8A6) Rabbit mAb is used as a loading control. | |
Immunohistochemical analysis of paraffin-embedded T-47D (left) and MDA-MB-231 (right) cell pellets using Prolactin Receptor (D4A9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human placenta using Prolactin Receptor (D4A9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Note the staining observed in placental decidual cells. |
