DKK1 (D5V6L) Rabbit mAb
货号：
48367S 基本售价：
3360.0 元 规格：
100ul
产品信息
概述| 货号 | 48367S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC |
| 目标/特异性 | DKK1 (D5V6L) Rabbit mAb recognizes endogenous levels of total DKK1 protein. |
| 使用方法 | WB(1:1000)
IP (1:100)
IF-IC (1:800) |
性能| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 28-40 |
| 参考文献 | 1 . Mao, B. et al. (2001) Nature 411, 321-5.
2 . Niehrs, C. (2006) Oncogene 25, 7469-81.
3 . Krupnik, V.E. et al. (1999) Gene 238, 301-13.
4 . Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
5 . Mao, B. et al. (2002) Nature 417, 664-7.
6 . Davidson, G. et al. (2002) Development 129, 5587-96.
7 . Glinka, A. et al. (1998) Nature 391, 357-62.
8 . Baron, R. and Rawadi, G. (2007) Curr Osteoporos Rep 5, 73-80.
9 . MacDonald, B.T. et al. (2007) Bone 41, 331-9.
10 . Diarra, D. et al. (2007) Nat Med 13, 156-63.
11 . Forget, M.A. et al. (2007) Br J Cancer 96, 646-53.
12 . Yamabuki, T. et al. (2007) Cancer Res 67, 2517-25.
13 . Kuphal, S. et al. (2006) Oncogene 25, 5027-36.
14 . Aguilera, O. et al. (2006) Oncogene 25, 4116-21.
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参考图片Western blot analysis of extracts of PC-3 and U-251 cells using DKK1 (D5V6L) Rabbit mAb. |
Confocal immunofluorescent analysis of PC-3 (positive, left) and U-2 OS (negative, right) cells using DKK1 (D5V6L) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
