| 货号 | 13171S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | RBBP5 (D3I6P) Rabbit mAb recognizes endogenous levels of total RBBP5 protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of RBBP5 (D3I6P) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 对4 x 106HeLa细胞中交联过的染色质进行染色质免疫沉淀，所用抗体为10 µl RBBP5 (D3I6P)或2 µl Normal Rabbit IgG #2729，富集到的DNA经过实时PCR定量，所使用产品为SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。 | |
Western blot analysis of extracts from various cell lines using RBBP5 (D3I6P) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为 RBBP5 (D3I6P) Rabbit mAb。 | |
Immunoprecipitation of RBBP5 from C2C12 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RBBP5 (D3I6P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RBBP5 (D3I6P) Rabbit mAb.从C2C12细胞提取物中免疫沉淀RBBP5，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或RBBP5 (D3I6P) Rabbit mAb (lane 3)。Lane 1为10% input。 使用RBBP5 (D3I6P) Rabbit mAb进行Western blot检测。 |
