| 货号 | 5104S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | RCC2 (D14F3) Rabbit mAb detects endogenous levels of total RCC2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC (reviewed in 1,2). In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B (3). RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell types using RCC2 (D14F3) Rabbit mAb. 使用RCC2 (D14F3) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中RCC2蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using RCC2 (D14F3) Rabbit mAb (green) and α-Tubulin (DM1A) Mouse mAb #3873 (red). 使用RCC2 (D14F3) Rabbit mAb (绿色)和α-Tubulin (DM1A) Mouse mAb #3873 (红色)标记，共聚焦免疫荧光分析HeLa细胞。 |
