| 货号 | 13069S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Ring1A (D2P4D) Rabbit mAb recognizes endogenous levels of total Ring1A protein. This antibody does not cross-react with Ring1B. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Ring1A plays a role in polycomb group (PcG) protein function. PcG proteins are critically involved in transcriptional repression of Hox genes during development (1,2). PcG proteins form two distinct complexes: EED-EZH2 and the PRC complex, which is composed of at least Bmi1 and Ring1A/Ring1B. The EZH2-containing complex is responsible for the methylation of H3K27, and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (3). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (4). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (5). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Confocal immunofluorescent analysis of HeLa cells using Ring1A (D2P4D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).采用共聚焦免疫荧光术检测Hela细胞，使用的抗体为Ring1A (D2P4D) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 鬼笔环肽进行标记（红色）。 Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from various cell lines using Ring1A (D2P4D) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为Ring1A (D2P4D) Rabbit mAb。 |
