货号 | 9355S |
反应种属 | Human/Mouse/Rat/Monkey/GuineaPig |
来源宿主 | Rabbit |
应用 | W/IP/IF-IC |
使用方法 | WB(1:1000) IP (1:25) IF-IC (1:25) |
供应商 | CST |
背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
存放说明 | -20C |
计算分子量 | 90 |
Western blot analysis of extracts from HeLa, L929 and AR42J cells, using RSK1/RSK2/RSK3 (32D7) Rabbit mAb.Western blot方法检测细胞提取物:HeLa, L929,AR42J细胞。使用的抗体是RSK1/RSK2/RSK3 (32D7) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells labeled with RSK1/RSK2/RSK3 (32D7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞,标记RSK1/RSK2/RSK3 (32D7) Rabbit mAb(呈绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记,呈红色。 |