| 货号 | 55920S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-F |
| 目标/特异性 | mGluR5 (D6E7B) Rabbit mAb recognizes endogenous levels of total mGluR5 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | mGluR5, a metabotropic glutamate receptor, is a class C G protein-coupled receptor that signals through the Gaq/11-PLC-inositol 1,4,5 triphosphate pathway (1). mGluR5 is comprised of a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular domain. Glutamate binding to mGluR5 leads to an increase in intracellular calcium levels and stimulation of PKC activity (2). In neurons, mGluR5 is found in the post-synapse, in a complex with NMDA receptors, PSD-95, SHANK, and Homer (3). mGluR5 is also expressed in microglia and astrocytes (4). Neuronal mGluR5 has been shown to interact with amyloid beta oligomers, and mGluR5 antagonists exhibit neuroprotective effects (5) placing mGluR5 as a potential therapeutic target for Alzheimer’s disease. In glial cells, mGluR5 appears to play an anti-inflammatory role by negatively regulating the release of inflammatory factors (6). |
| 存放说明 | -20C |
| 计算分子量 | 150, 300 |
| 参考文献 | 1 . Abdul-Ghani, M.A. et al. (1996) J Neurophysiol 76, 2691-700. 2 . Pin, J.P. and Duvoisin, R. (1995) Neuropharmacology 34, 1-26. 3 . Tu, J.C. et al. (1999) Neuron 23, 583-92. 4 . Verkhratsky, A. et al. (1998) Physiol Rev 78, 99-141. 5 . Bruno, V. et al. (2000) Neuropharmacology 39, 2223-30. 6 . Byrnes, K.R. et al. (2009) Glia 57, 550-60. |
Immunoprecipitation of mGluR5 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is mGluR5 (D6E7B) Rabbit mAb. Western blot analysis was performed using mGluR5 (D6E7B) Rabbit mAb. | |
Western blot analysis of extracts from the indicated tissues using mGluR5 (D6E7B) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Confocal immunofluorescent analysis of mouse hippocampus (left) and striatum (right) using mGluR5 (D6E7B) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
