| 货号 | 5529S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | S100A10 (4E7E10) Mouse mAb recognizes endogenous levels of total S100A10 protein. This antibody is not known to react with other S100 family proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners that suggest each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). |
| 存放说明 | -20C |
| 计算分子量 | 11 |
Confocal immunofluorescent analysis of HEK001 cells using S100A10 (4E7E10) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HEK001细胞使用S100A10 (4E7E10)鼠单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 | |
Flow cytometric analysis of Jurkat cells (blue) and HEK001 cells (green) using S100A10 (4E7E10) Mouse mAb. 对Jurkat细胞（蓝色）和HEK001细胞（绿色）使用S100A10 (4E7E10)鼠单抗进行共聚焦免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded human prostate using S100A10 (4E7E10) Mouse mAb. 对石蜡包埋的人前列腺使用S100A10 (4E7E10)鼠单抗进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using S100A10 (4E7E10) Mouse mAb. 对多个细胞系使用S100A10(4E7E10)鼠单克隆抗体进行Western blot分析。 |
