| 货号 | 2217S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-F/IF-IC |
| 目标/特异性 | S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody detects endogenous levels of total S6 ribosomal protein independent of phosphorylation. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析HeLa、NIH/3T3、PC12和COS细胞中S6 Ribosomal Protein(5G10)水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片，结果显示S6 Ribosomal Protein (5G10)定位在细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫组化分析人类结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫组化分析人类Non-Hodgkins lymphoma组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫组化分析人类乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗，免疫组化分析人类前列腺癌组织石蜡切片。 | |
Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (5G10) Rabbit mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 与一个sotype control (右图)比较，使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 (红色, 左图)标记，共聚焦免疫荧光观察S6 Ribosomal Protein蛋白在HeLa细胞中定位。fluorescein phalloidin标记微丝蛋白。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Confocal immunofluorescent analysis of mouse brain using S6 Ribosomal Protein (5G10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 (绿色) 和GFAP (GA5) Mouse mAb鼠单抗 #3670 (红色)标记，共聚焦免疫荧光观察S6 Ribosomal Protein蛋白在小鼠大脑组织中定位。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 |
