| 货号 | 2996S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | SET8 (C18B7) Rabbit mAb detects endogenous levels of total SET8 protein (both isoforms). |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1-3). SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates (1-3). SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase (4,5). SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase (5-7). Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle (6,7). Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53 (8). In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis (8). Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase. |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb. 使用SET8 (C18B7) Rabbit mAb，免疫印迹(Western blot)分析HCT116和RAW细胞中SET8 (C18B7)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with 0.5% Triton X-100 for 10 minutes prior to fixation according to Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (right), using SET8 (C18B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用SET8 (C18B7) Rabbit mAb (绿色)，共聚焦免疫荧光分析HeLa细胞，细胞分为未处理 (左图)或在固定之前处理0.5% Triton X-100 for 10 minutes依据Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (右图)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5™ (DNA荧光染料)。 |
