| 货号 | 8469S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | SirT1 (1F3) Mouse mAb recognizes endogenous levels of total SirT1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from various cell lines using SirT1 (1F3) Mouse mAb. 使用SirT1 (1F3) Mouse mAb鼠单抗，免疫印迹(Western blot)分析不同细胞中SirT1 (1F3)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa (left) and C2C12 (right) cells using SirT1 (1F3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用SirT1 (1F3) Mouse mAb 鼠单抗(绿色)标记，共聚焦免疫荧光分析HeLa (左图)和C2C12 (右图)细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Confocal immunofluorescent analysis of MEF WT (left, positive) and MEF SirT1 KO (right, negative) cells usingSirT1 (1F3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School. | |
Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryo fibroblasts (MEF) using SirT1 (1F3) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School. |
