| 货号 | 9475S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | SirT1 (D1D7) Rabbit mAb recognizes endogenous levels of total SirT1 protein. This antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Confocal immunofluorescent analysis of HeLa (positive; left), WT MEF (positive; middle), and SirT1 KO MEF (right) cells using SirT1 (D1D7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.采用共聚焦免疫荧光术检测Hela细胞（阳性，左）、WT MEF细胞（阳性，中）以及SirT1 KO MEF 细胞(右) ，使用的抗体为T SirT1 (D1D7) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 鬼笔环肽进行标记（红色）。WT 和KO MEF 由Wenyi Wei, Harvard Medical School惠赠 。 | |
Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为SirT1 (D1D7) Rabbit mAb。 | |
Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT1 (D1D7) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.Western blot方法检测SirT6 wild-type (WT) 和 knockout (KO) 小鼠胚胎成纤维细胞(MEF)的提取物，使用的抗体为SirT1 (D1D7) Rabbit mAb (上图)或 β-Actin （D6A8） Rabbit mAb #8457 （下图）。WT 和 KO MEF 由Wenyi Wei, Harvard Medical School惠赠。 |
