| 货号 | 2627S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | SirT3 (C73E3) Rabbit mAb detects endogenous levels of SirT3 protein, both the uncleaved 45 kDa precursor protein and the cleaved 28 kDa protein. The antibody does not cross-react with other sirtuins. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT3, a mammalian homolog of Sir2, exists in human cells in two forms. The full-length 44 kDa protein localizes to the nucleus, while a processed 28 kDa protein lacking 142 amino terminal residues localizes exclusively to the mitochondria (2-4). The single murine form of SirT3 is equivalent to the processed human SirT3 protein (2). Full-length SirT3 protein is processed in the mitochondrial matrix by the mitochondrial matrix processing peptidase (MMP) (3). Both full-length and processed forms of SirT3 are enzymatically active and de-acetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro (2). SirT3 also de-acetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria (5). Restricted caloric intake, which is linked to increased lifespan in multiple organisms, increases SirT3 expression in white and brown adipocytes of obese mice, suggesting a role for SirT3 in aging (6). Two observations implicate SirT3 in the regulation of mitochondrial thermogenesis. First, exposure to cold temperatures increases SirT3 expression in brown adipocytes, while elevated temperatures reduce SirT3 expression (6). Second, over-expression of SirT3 results in increased levels of the mitochondrial uncoupling protein 1 (UCP1) (6). SirT3 protein levels are also elevated in certain breast cancers (7). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of various cell extracts using SirT3 (C73E3) Rabbit mAb. 使用SirT3 (C73E3) Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中SirT3蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SirT3 (C7E3) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). 使用SirT3 (C7E3) Rabbit mAb兔单抗，其分别与对照肽段 (左图)或抗原特异性肽段 (右图)孵育，免疫组化分析人源乳腺癌组织石蜡切片。 |
