| 货号 | 2954S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Smac/Diablo Mouse mAb detects endogenous levels of processed and unprocessed human Smac/Diablo. The antibody reacts weakly with mouse and rat Smac/Diablo. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Smac/Diablo Mouse mAb (left) or secondary alone (right). 免疫组织化学分析石蜡包埋的人类肾细胞癌切片，使用抗体为Smac/Diablo Mouse mAb 鼠单抗（左）或单独的二抗（右）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Smac/Diablo Mouse mAb. 免疫组织化学分析石蜡包埋的人乳腺癌切片，使用抗体为Smac/Diablo Mouse mAb鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Smac/Diablo Mouse mAb. 免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为Smac/Diablo Mouse mAb 鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Smac/Diablo Mouse mAb. 免疫组织化学分析石蜡包埋的人肺癌切片，使用抗体为Smac/Diablo Mouse mAb鼠单抗。 | |
Western blot analysis of extracts from 293, HeLa and Jurkat cells, using Smac/Diablo Mouse mAb. Western blot方法检测293、HeLa和Jurkat 细胞提取物，使用抗体为Smac/Diablo Mouse mAb鼠单抗。 | |
Confocal immunofluorescent analysis of MCF-7 cells, using Smac/Diablo Mouse mAb (left, green) or with MitoTracker® Red CMXRos (right, red), which stains mitochondria, demonstrating colocalization. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析MCF-7细胞，使用Smac/Diablo Mouse mAb 鼠单抗（左，绿色）或MitoTracker® Red CMXRos（右，红色），其线粒体染色说明了共定位情况。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
