| 货号 | 12760S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | SMARCC2/BAF170 (D8O9V) Rabbit mAb recognizes endogenous levels of total SMARCC2/BAF170 protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). |
| 存放说明 | -20C |
| 计算分子量 | 162, 170 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells, grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min), and either 10 μl of SMARCC2/BAF170 (D8O9V) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 MCF7细胞中提取的交联过的染色质，以及10 µl SMARCC2/BAF170 (D8O9V) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀，这些细胞需要在含5%活性炭处理胎牛血清以及无酚红的培养基中培养4天，之后再用β-estradiol 进行处理(10 nM, 45 min)。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量，所使用引物为SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, 以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。 | |
Western blot analysis of extracts from various cell lines using SMARCC2/BAF170 (D8O9V) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为SMARCC2/BAF170 (D8O9V) Rabbit mAb。 | |
Immunoprecipitation of SMARCC2/BAF170 from PANC-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SMARCC2/BAF170 (D8O9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SMARCC2/BAF170 (D8O9V) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀SMARCC2/BAF170，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或SMARCC2/BAF170 (D8O9V) Rabbit mAb (lane 3)。Lane 1为10% input。 使用SMARCC2/BAF170 (D8O9V)进行Western blot检测。 |
