| 货号 | 61702S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP ChIP |
| 目标/特异性 | SET1A (D3V9S) Rabbit mAb recognizes endogenous levels of total SET1A protein. This antibody does not cross-react with other SET1/COMPASS proteins, including SET1B, MLL1, MLL2, MLL3, and MLL4. |
| 使用方法 | WB(1:1000) IP (1:200) ChIP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6). |
| 存放说明 | -20C |
| 计算分子量 | 300 |
| 参考文献 | 1 . Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7. 2 . Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8. 3 . Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36. 4 . Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31. 5 . Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28. 6 . Hughes, C.M. et al. (2004) Mol Cell 13, 587-97. |
Immunoprecipitation of SET1A from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SET1A (D3V9S) Rabbit mAb. Western blot analysis was performed using SET1A (D3V9S) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 5 μL of SET1A (D3V9S) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human β-Actin Promoter Primers #13653, SimpleChIP®Human GAPDH Promoter Primers #4471, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from 293T and HeLa cells using SET1A (D3V9S) Rabbit mAb. |
