| 货号 | 5765S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | SRC-3 (D1F11) Rabbit mAb recognizes endogenous levels of total SRC-3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:600) |
| 供应商 | CST |
| 背景 | There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Confocal immunofluorescent analysis of MCF7 (left) and 293 (right) cells using SRC-3 (D1F11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用SRC-3 (D1F11) Rabbit mAb 兔单抗(绿色)标记，共聚焦免疫荧光分析MCF7 (左图)和293 (右图)细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using SRC-3 (D1F11) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 使用SRC-3 (D1F11) Rabbit mAb兔单抗 (上图)或β-Actin (D6A8) Rabbit mAb #8457兔单抗 (下图)，免疫印迹(Western blot)分析不同细胞中SRC-3蛋白水平。 |
